RNA Preparation and Purification
Research Products International Corp Direct-zol RNA MiniPrep Plus w/TRI Reagent, & Zymo-Spin IIICG Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Epoch Life Science Inc GenCatch™ Total RNA Extraction System (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from various starting materials (such as cultured cells, tissues, bacteria, yeast, etc.). Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 65 ug yield. 200 ul elution volume. No phenol/chloroform extraction nor ethanol precipitation required. Instructions for sample type listed in protocol. Used by labs around the world. 18 month shelf life.
Norgen Biotek Corp Total Rna Purificatio 250Preps
This kit is suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
MyBioSource RNA (For fixed/embedded tissues)(without Phenol & Chloroform, need not DNase I digestion, directly use in RT-PCR or qPCR) Isolation Kit, 50 Tests
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 5 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep with Sample Purification Beads – 24 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg. For convenience, this kit includes SPRISelect beads.
New England Biolabs, Inc. Monarch® Total RNA Miniprep Kit - 50 preps
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Total RNA, including viral RNA, can also be extracted from clinically relevant samples like saliva and swabs. Cleanup of enzymatic reactions or purification of RNA from TRIzol-extracted samples is also possible using this kit. Purified RNA is highly pure, has minimal residual gDNA and ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, RNA-seq, Northern blot analysis, etc.
Norgen Biotek Corp CYTOPLASMIC/NCLR RNA PURIF KIT
Cytoplasmic & Nuclear RNA Purification Kit,100 Preps
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Bone Marrow, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Parietal Lobe, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Thalamus, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Tumor Cell Line: Jurkat, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Tumor Cell Line: MCF 7, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.